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Dina Mahmoud Ahmed Hasanin

Associate Professor

Faculty member

كلية العلوم
Building 5,3rd floor,office 114
المنشورات
مقال فى مجلة
2020

Luteolin protects against testicular injury induced by lead acetate by activating the Nrf2/HO-1 pathway

El-Khadragy, Wafa A. AL-Megrin, Suliman Alomar, Afrah F. Alkhuriji, Dina M. Metwally, Shimaa K. Mohamed, Rami B. KassabAhmed E. Abdel Moneim, Manal F. . 2020

Lead (Pb) is a toxic heavy metal that is harmful to humans, especially male reproductive organs. Luteolin (LUT) is a naturally occurring flavonoid with numerous biological activities. Our aim was to investigate the possible reproprotective effect of LUT against testicular deficits induced by Pb intoxication. In the present study, 28 rats were distributed into 4 groups: control, LUT (50 mg/kg), lead acetate (PbAc, 20 mg/kg), and LUT + PbAc groups, in which rats were pre-treated with LUT 3 hr before PbAc injection. All animals were treated for 7 days. Oxidative stress, inflammatory and apoptotic markers along with histopathological changes have been examined using spectrophotometric, ELISA, real-time PCR, and histopathological methods. PbAc injection elevated Pb concentration in testicular tissue and decreased levels of sex hormones. PbAc intoxication exacerbated lipoperoxidation and nitric oxide formation, depleted superoxide dismutase, and catalase activities along with glutathione and its originated enzymes (glutathione peroxidase and glutathione reductase). At the molecular level, PbAc deactivated nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 in the testicular tissue. In addition, PbAc toxicity induced inflammatory and apoptotic cascades in testicular tissue as evidenced by the increased tumor necrosis factor-alpha, interleukin-1 beta, inducible nitric oxide synthase, Bax, and caspase 3, while Bcl-2 was declined. Histopathological examination of testicular tissue also revealed that PbAc caused degeneration alterations in spermatogenic cells, the spermatogenic epithelial cells were disconnected from the basement membrane, and the seminiferous tubules were vacuolated. Remarkably, pre-treatment with LUT minimized significantly the testicular damage induced by PbAc. Therefore, we conclude that LUT may have a beneficial effect against PbAc-induced testicular injury through preventing oxidative challenge, inflammation, and finally apoptosis.

الصفحات
12
مزيد من المنشورات
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Malaria is an important health problem in subtropical and tropical areas around the world. Infection with protozoan parasites of the Plasmodium genus, which grow inside host erythrocytes, causes…

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Sarcocystis spp. are intracellular protozoan parasites with an intermediate-definitive host life cycle based on a prey–predator relationship. Sarcocystis infection is common among different…

2021
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Arsenic is a potent neurotoxic xenobiotic found in drinking water, and food. Chlorogenic acid (CGA), an abundant polyphenol in coffee, potentially has a wide range of therapeutic benefits.

بواسطة Dina M. Metwallya,b,c , Reem A. Alajmia , Manal F. El-Khadragyd,e,⁎ , Hany M. Yehiaf,g , Wafa A. AL-Megrind , Ahmed M.A. Akabawyh , Hatem K. Aminh , Ahmed E. Abdel Moneim
2020