Production of monoclonal antibody, Anti-CD3 by hybridoma cells cultivated in basket spinner under free and immobilized conditions
Journal Article
HA, Elsayed EA, Abou-Shleib H, Omar H, El-Demellawy M, Shahin H, Wadaan MA, El-Enshasy . 2013
Magazine \ Newspaper:
BMC Proceedings
Issue Number:
Suppl 6
Volume Number:
7
Pages:
1-5
Publication Abstract:
Monoclonal antibodies (Mabs) have been recently used for the treatment of virtually every debilitating disease. Packed-bed bioreactors have been used for the cultivation and production of a wide range of cell lines and biologics including MAbs. The principle behind a Packed-bed bioreactor is that the cells are being immobilized within a suitable stationary matrix which is represented by the bed. Packed-bed bioreactors also have the advantage of being capable of generating high cell densities with a low concentration of free cells in suspension; hence, simplifying downstream processing. The present work was designed to compare between the cultivation of hybridoma cells as well as the production of OKT3 MAb in free and immobilized culture conditions