Production of monoclonal antibody, Anti-CD3 by hybridoma cells cultivated in basket spinner under free and immobilized conditions
HA, Elsayed EA, Abou-Shleib H, Omar H, El-Demellawy M, Shahin H, Wadaan MA, El-Enshasy . 2013
Monoclonal antibodies (Mabs) have been recently used for the treatment of virtually every debilitating disease. Packed-bed bioreactors have been used for the cultivation and production of a wide range of cell lines and biologics including MAbs. The principle behind a Packed-bed bioreactor is that the cells are being immobilized within a suitable stationary matrix which is represented by the bed. Packed-bed bioreactors also have the advantage of being capable of generating high cell densities with a low concentration of free cells in suspension; hence, simplifying downstream processing. The present work was designed to compare between the cultivation of hybridoma cells as well as the production of OKT3 MAb in free and immobilized culture conditions
Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4…
A series of azocompounds containing methyl salicylate 4a–k and 1-naphthyl moiety 6–8 was synthesized and tested as anticancer
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Invertase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) constitutes an improtant microbial enzyme with wide applications in differnt food and pharmaceutical sectors. The present work used…