Improvement of cell mass production of Lactobacillus delbrueckii sp. bulgaricus WICC-B-02: A newly isolated probiotic strain from mother’s milk

Journal Article

Enshasy, Elsayed A. Elsayed, Nor Zalina Othman, Roslinda Malek,Tebbie Tang, and HeshamA. El . 2014

• Magazine \ Newspaper: J. of Appl. Pharm. Sci
• Issue Number: 11
• Volume Number: 4
• Pages: 8-14

Publication Abstract

Lactobacillus delbrueckii  sp. bulgaricus  WICC-B-02  is new probiotic strain which  was initially isolated from the mother’s milk.  As lactic acid bacterium, it’s known as a  highly efficient  probiotic microorganism with a wide range of benefits on the human health. This study was conducted to design and establish industrial platform for high  cell  mass  production  of  L.  delbrueckii  sp.  bulgaricus  for  pharmaceutical  and  food  industries  application. However, due to  low  cell  mass production caused by the accumulation of  lactic acid during  the  cultivation of lactic  acid  bacteria,  therefore,  the  optimization  of  cell  mass  production  with  low  lactic  acid  production  was developed in this study.  The new formulation of the production medium was developed by optimizing  the main components such as glucose  (30 g.L-1), yeast extract (6.0 g.L-1) and peptone  (6.0 g.L-1) in shake flask cultivation. The  cell  mass  production  was  3.14  g.L-1,  and  it  increased  to  6.08g.L-1 with  the  lactic  acid  production  being  reduced by about 33%, compared to the un-optimized medium