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Dr. rer. nat Elsayed Ahmed Elsayed

Associate Professor

Faculty Staff Member

كلية العلوم
Building 5, AB107
المنشورات
مقال فى مجلة
2018
تم النشر فى:

Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system

M, El Enshasy, Elsayed EA, Suhaimi N, MalekRA, Esawy . 2018

Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020. Results: The production medium was first optimized using a statistical optimization approach to increase pectinase production. A maximal enzyme concentration of 76.35 U/mL (a 2.8-fold increase compared with the initial medium) was produced in a medium composed of (g/L): pectin, 32.22; (NH4)2SO4, 4.33; K2HPO4, 1.36; MgSO4.5H2O, 0.05; KCl, 0.05; and FeSO4.5H2O, 0.10. The cultivations were then carried out in a 16-L stirred tank bioreactor in both batch and fed-batch modes to improve enzyme production, which is an important step for bioprocess industrialization. Controlling the pH at 5.5 during cultivation yielded a pectinase production of 109.63 U/mL, which was about 10% higher than the uncontrolled pH culture. Furthermore, fed-batch cultivation using sucrose as a feeding substrate with a rate of 2 g/L/h increased the enzyme production up to 450 U/mL after 126 h. Conclusions: Statistical medium optimization improved volumetric pectinase productivity by about 2.8 folds. Scaling-up the production process in 16-L semi-industrial stirred tank bioreactor under controlled pH further enhanced pectinase production by about 4-folds. Finally, bioreactor fed-batch cultivation using constant carbon source feeding increased maximal volumetric enzyme production by about 16.5-folds from the initial starting conditions.

رقم المجلد
18
مجلة/صحيفة
BMC Biotechology
الصفحات
1-13
مزيد من المنشورات
publications

Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4…

بواسطة El Enshasy, Elsayed EA, Suhaimi N, MalekRA, Esawy M
2018
publications

A series of azocompounds containing methyl salicylate 4a–k and 1-naphthyl moiety 6–8 was synthesized and tested as anticancer
agents. Nitrosation of methyl 5-amino-2-hydroxybenzoate or 1-…

بواسطة El-Naggar M, El-Shorbagi A-N, El-Naggar D, Amr AE, Al-Omar M, Elsayed EA
2018
publications

Invertase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) constitutes an improtant microbial enzyme with wide applications in differnt food and pharmaceutical sectors. The present work used…

بواسطة Elsayed EA, El Enshasy HA
2018