Isolation and characterization of collagen from the skin, scales and fins of Cyrinus carpio and Hypophthalmichthys molitrix
The study was designed to isolate and characterize the collagen from skin, scales and fins of Cyprinus carpio and
Hypophthalmichthys molitrix. For this purpose nine fishes of each species of three weight groups were collected and
categorized as W1(250-500 g), W2(501-750 g) and W3(751- 1000 g). The maximum skin protein content was recorded
as 28.13% and 29.13% in C. carpio and H. molitrix, respectively, from the W3. The scales of the W3displayed a
maximum protein contents as 29.91 and 28.38% for C. carpio and H. molitrix, respectively. The maximum protein
contents (19.19% and 21.37% for C. carpio and H. molitrix, respectively) were observed in the W3group. Protein
characterisation using SDS-PAGE revealed the molecular weights (kDa) of the C. carpio skin, scales, and fins ranged
from 126 kDa to 220 kDa, 78 kDa to 211 kDa, and 72 kDa to 141 kDa, respectively. The size of the collagen proteins of
the H. molitrix skin, scales and fins ranged from 117 kDa to 208 kDa, 81 kDa to 219 kDa, and 72 kDa to 141 kDa,
respectively. Glycine was the most abundant amino acid, whereas, Tryptophan was totally absent in all selected tissues.
Significant differences were observed for amino acids within the weight groups and between the two fish species
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