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فهد عبده أحمد المخلافي

Associate Professor

قسم علم الحيوان

كلية العلوم
مبنى 5 الدور الأرضي رقم المكتب أب 75/1
المنشورات
مقال فى مجلة
2016

Larvicidal Activities of Indigenous Bacillus thuringiensis Isolates and Nematode Symbiotic Bacterial Toxins against the Mosquito Vector, Culex pipiens (Diptera: Culicidae)

Background: The incidence of mosquito-borne diseases and the resistance of mosquitoes to conventional pesticides
have recently caused a panic to the authorities in the endemic countries. This study was conducted to identify native
larvicidal biopesticides against Culex pipiens for utilization in the battle against mosquito-borne diseases.
Methods: Larvicidal activities of new indigenous Bacillus thuringiensis isolates and crude toxin complexes (TCs) of
the two nematode bacterial-symbionts, Photorhabdus luminescens akhurstii (HRM1) and Ph. luminescens akhurstii
(HS1) were tested against Cu. pipiens. B. thuringiensis isolates were recovered from different environmental samples
in Saudi Arabia, and the entomopathogenic nematodes, Heterorhabditis indica (HRM1) and He. sp (HS1) were isolated
from Egypt. Larvicidal activities (LC50 and LC95) of the potentially active B. thuringiensis strains or TCs were
then evaluated at 24 and 48h post-treatment.
Results: Three B. thuringiensis isolates were almost as active as the reference B. thuringiensis israelensis (Bti-H14),
and seven isolates were 1.6–5.4 times more toxic than the Bti-H14. On the other hand, the TCs of the bacterial symbionts,
HRM1 and HS1, showed promising larvicidal activities. HS1 showed LC50 of 2.54 folds that of HRM1 at 24h
post-treatment. Moreover, histopathological examinations of the HS1-treated larvae showed deformations in midgut
epithelial cells at 24h post-treatment.
Conclusion: Synergistic activity and molecular characterizations of these potentially active biocontrol agents are
currently being investigated. These results may lead to the identification of eco-friend mosquito larvicidal product(s)
that could contribute to the battle against mosquito-borne diseases.

رقم المجلد
11
مجلة/صحيفة
J Arthropod Borne Dis
الصفحات
260-277
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