Evaluating Developmental Toxicity of Solvent Fractions Obtained from Pulicaria glutinosa on Zebrafish Embryo and their Antimicrobial Potential

Journal Article

Wadaan, 12. Nael Abutaha1, Muhammad Farooq, Fahd Abdu Al-Mekhlafi, Baabbad Almohannad, Sabir Shehata and Mohammad . 2014

• Publication Work Type: Research article
• Publication Online URL: Evaluating Developmental Toxicity of Solvent Fractions Obtained from Pulicaria glutinosa on Zebrafish Embryo and their Antimicro
• Tags: Pulicaria glutinosa, Danio rerio, antioxidant, neurotoxicity.
• Magazine \ Newspaper: JOURNAL OF PURE AND APPLIED MICROBIOLOGY
• Issue Number: 4
• Volume Number: 8
• Pages: 2679-2685

Publication Abstract

Pulicaria genus belongs to the family Compositae. Some species of this genus have been used in traditional Arab medicine as antispasmodic agent. In this study the Pulicaria glutinosa, which is a wild growing shrub in Saudi Arabia have been explored for developmental toxicity using zebrafish embryos. The fractionated crude extract showed diverse biological activity as methanol fraction exhibited highest antioxidant activity (IC50 12.2?0.27%) followed by chloroform fraction (18.1?0.36%), while hexane fraction did not show any antioxidant activity. Treating zebrafish (Danio rerio) embryos with fractionated extracts of Pulicaria glutinosa revealed that hexane fraction induced severe neurotoxicity in growing zebrafish embryos even at very low concentrations (5?g/ml), while methanol fraction which had highest antioxidant activity did not produce any teratogenic profile even at 100?g/ml. This is the first report to evaluate the fresh leaves of Pulicaria glutinosa for antimicrobial, antioxidant activity and developmental toxicity in zebrafish embryos