Combination of Vitamin E and Lactobacillius plantarum Reverses Mercuric Chloride-Induced Neurotoxicity: Implication of BDNF, CREB and MAPK Proteins Expressions

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Fadda, Laila M . 2020
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Abstract Mercury is the third most hazardous heavy metal and its toxicity causes a severe health risk through unfavorable detrimental pathological and biochemical effects. Mercury is widely found in many ecological and certain occupational settings. Objectives:  The aim of this study is to elucidate the neuroprotective role of vitamin E (VE)  and Lactobacillus plantarum (LTB)  either alone or in combination against a toxic sublethal dose of Mercuric chloride (MC). Methods: First group served as a normal control group; rats from the second group were intoxicated with( 5 mg/kg MC once daily); the third group was treated with VE; the fourth group was treated with LTB;  and the fifth group was treated with VE and LTB. All treatments were given daily along with MC for fourteen days. Results: The results of the current study confirmed that MC prompted an elevation in serum TNF-α, IL-6 and brain  lipid peroxides , protein expression of mitogen-activated protein kinase  (MAPK) and mRNA expression of Bax  and caspase -3 level  as well as DNA degradation. However, Brain-derived neurotrophic factor (BDNF) and cAMP response element-binding (CREB) protein expressions,  GSH level and SOD activity were down-regulated.
The intake of LTB and /or VE along with MC intoxication significantly mitigated the  alteration in all the previous parameters. Moreover, histopathology analysis of brain sections confirmed MC- induced brain injury and that LTB or VE alone or together were capable of ameliorating brain artitechture. Conclusions: The combination of LTB and VE was an  effective therapy in the management of MC-induced neuroioxicity  and this combination can be considered a useful therapeutic candidate against brain injury induced by MC. BDNF, MAPK and CREB protein expressions are implicated in MC -induced brain injury and its treatment..

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