Assistant Professor | Researcher

Address: Department of Food Sciences, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia | Phone: lab extension no 79094

How methyl cyanide induces aggregation in all-alpha proteins: A case study in four albumins

Journal Article
Khan, Priyankar Sen, Sadaf Fatima, Javed Masood Khan, Rizwan Hasan . 2009
Issue Number: 
2
Volume Number: 
44
Pages: 
163-169
Publication Abstract: 

Serum albumins are chief carrier of ligands in blood, hence important in clinical biotechnology. The effects of methyl cyanide (MeCN), a chief solvent of reverse phase chromatography, on four mammalian serum albumins (human, bovine, porcine and rabbit sources) were studied at neutral pH with the help of scattering, circular dichroism, IR and fluorescence spectroscopy. We have detected an intermediate state in the presence of 20% (v/v) MeCN, having 8–9% higher α-helical structure than that of their native states. In the presence of 60% (v/v) MeCN another intermediate was observed with non-native β-sheet structure and high tendency to form aggregates.