Bactericidal and Antibiotics synergistic effect of Nanosilver against Methicillin-Resistant staphylococcus aureus.
Journal Article
AbdelRahim, Khalid . 2015
Publication Work Type:
بحث
Publication Online URL:
Magazine \ Newspaper:
Jundishapur Journal of Microbiology
Issue Number:
11
Volume Number:
8
Pages:
DOI: 10.5812/jjm.25867
Publication Abstract:
Bachground :Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for several difficult-to-treat infections in humans. MRSA strains has developed, through the process of natural selection, resistance to beta-lactame antibiotics. MRSA infection are more difficult to be treated with standard types of antibiotics and thus more dangerous to human health.
Objectives : To evaluate the bactericidal and antibiotic synergistic effect of silver Nanoparticles (Ag-NPs)product, against MRSA.
Material and Methods: MRSA was isolated from clinical samples and identified and susceptibility tested using MicroScan® WalkAway-96® SI System. MIC was determined by a microdilution method. Time kill assay was determined by exposing MRSA to different concentrations of Ag-NPs and monitoring bacterial growth by measuring optical density at 600nm. Tissue culture plate was used for determination of efficacy of Ag-NPs and their combination with antibiotics in the elimination of formed biofilm .
Results : The MIC value of Ag -NPs against MRSA was in at of 100 μg/ml. MRSA cells were treated with 50, 100 and 200 µg/ml Ag-NPs inhibited bacterial growth and after 4 h, almost all treated MRA cells were dead. All combinations showed effectiveness against the MRSA. It was observed that MRSA did not show inhibition zone with ampicillin alone.