Ethanol Extract of Cissus quadrangularis Enhances Osteoblast Differentiation and Mineralization of Murine Pre-Osteoblastic MC3T3-E1 Cells
SHAKOORI, RAAZIA TASADDUQ,1,2 JONATHAN GORDON,2 KHALID A. AL-GHANIM,3 JANE B. LIAN,2 ANDRE J. VAN WIJNEN,4 JANET L. STEIN,2 GARY S. STEIN,2 AND ABDUL RAUF . 2017
Traditional medicinal literature and previous studies have reported the possible role of Cissus quadrangularis (CQ) as an anti-osteoporotic
agent. This study examines the effectiveness of CQin promoting osteoblast differentiation of the murine pre-osteoblast cell line,MC3T3-E1.
Ethanolic extract ofCQ(CQ-E) was found to affect growth kinetics ofMC3T3-E1 cells in a dosage-dependentmanner. High concentrations of
CQ-E (more than 10mg/ml) have particularly adverse effects, while lower concentrations of 0.1 and 1mg/ml were non-toxic and did not affect
cell viability. Notably, cell proliferation was significantly increased at the lower concentrations of CQ-E. CQ-E treatment also augmented
osteoblast differentiation, as reflected by a substantial increase in expression of the early osteoblast marker ALP activity, and at later stage, by
mineralization of extracellular matrix compared to the control group. These findings suggest dose-dependent effect of CQ-E with lower
concentrations exhibiting anabolic and osteogenic properties.
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