Portable hybrid gamma-near-infrared fluorescence imaging
Conference Paper
Bugby, S.L. . 2016
نوع عمل المنشور:
e-poster
رابط المنشور على الويب:
اسم المؤتمر:
European Association of Nuclear Medicine EANM 2016
عنوان المؤتمر:
Barcelona, Spain
تاريخ المؤتمر:
السبت, تشرين اﻷول (أكتوبر) 15, 2016
المنظمة الراعية:
EANM
مستخلص المنشور:
Introduction
The first clinical study of targeted hybrid near-infrared (NIR) fluorescent-radioactive tracers was recently published. In addition, ICG-99mTc-nanocolloid is already being used by some centres for sentinel lymph node biopsy. Once exposed, the NIR fluorescence reporter can be imaged at very high resolutions while the radioactive component allows imaging at depths which would not be possible in NIR. Visualisation of NIR fluorescence during surgery requires a dedicated NIR camera, several of which are available commercially. Gamma detection may be carried out with a separate portable gamma camera or with a non-imaging probe.
We describe a portable hybrid NIR-gamma small field of view camera, capable of displaying co-aligned images from both modalities which can be fused into one image or viewed separately. This study is a preliminary investigation of the performance of the fluorescence component of this camera, including phantom studies and first images from a preclinical pilot study.
Materials and Methods
The hybrid camera consists of a 1.5mm thick CsI:Tl columnar scintillator coupled to an electron multiplying CCD. A 1.0mm diameter pinhole collimator gives a 40mm x 40mm nominal field of view for an 8mm x 8mm detector area. A fluorescence camera was aligned to provide the same field of view as the gamma camera with an LED ring as the excitation source. The performance of the fluorescence imaging was quantified in this study for the fluorophores ICG and IRDye800CW (CW800) using a number of phantom experiments. Images are also presented for a preclinical study of a targeted hybrid tracer (cRGD-CW800-TCO + TCO-DOTA-111In) in mice with HT29 colorectal cancer xenografts. These images have been used for a qualitative comparison between the hybrid camera and commercially available fluorescence imaging systems (Artemis, Quest; Pearl Impulse, LICOR).
Results and conclusions
The hybrid camera prototype has been shown to successfully image the dual NIR-gamma tracers tested. With further development, this camera could be used intraoperatively, offering the benefits of high resolution surface NIR fluorescence imaging and the depth penetration of gamma imaging in a single imaging system.