SYNTHETIC SEED PRODUCTION AND PHYSIO-BIOCHEMICAL STUDIES IN CASSIA ANGUSTIFOLIA VAHL. – A MEDICINAL PLANT

Journal Article

, Najat A. Bokhari . 2014

• Publication Work Type: بحث منشور
• Magazine \ Newspaper: Acta Biologica Hungarica ISSN 0236-5383
• Issue Number: 65
• Volume Number: (Vol. 65 (3
• Pages: 355–367

Publication Abstract

Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of

cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of

C. angustifolia

in

calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl

2

∙

2H

2

O were found most suitable for

encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and Skoog medi

-

um supplemented with thidiazuron (5.0 μM) + indole-3-acetic acid (1.0 μM) produced maximum number

of shoots (10.9

±

0.78) after 8 weeks of culture exhibiting (78%)

in vitro

conversion response.

Encapsulated nodal segments demonstrated successful regeneration after different period (1–6 weeks) of

cold storage at 4 °C. The synthetic seeds stored at 4 °C for a period of 4 weeks resulted in maximum

conversion frequency (93%) after 8 weeks when placed back to regeneration medium. The isolated shoots

when cultured on half strength Murashige and Skoog medium supplemented with 1.0 μM indole-3-butyr

-

ic acid (IBA), produced healthy roots and plantlets with well-developed shoot and roots were success

-

fully hardened off in plastic pots containing sterile soilrite inside the growth chamber and gradually

transferred to greenhouse where they grew well with 85% survival rate. Growth performance of 2 months

old

in vitro

-raised plant was compared with

in vivo

seedlings of the same age. Changes in the content of

photosynthetic pigments, net photosynthetic rate (P

N

), superoxide dismutase and catalase activity in

C. angustifolia

indicated the adaptation of micropropagated plants to

ex vitro

conditions