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منال فوزي محمود الخضرجي

Associate Professor

عضو هيئة التدريس

كلية العلوم
T56
المنشورات
فرضية
2013

Cloning, sequencing and expressing of the carotenoid biosynthesis genes of ß-carotene from epiphytic bacteria Erwinia uredovora in non carotenogenic bacteria E. coli.

Abstract: Four genes which encode the enzymes of ß-carotene in the epiphytic bacteria Erwinia uredovora have
been designated as crtE, crtB, crtI and crtY. These genes were cloned in Eschershia coli BL21 and located on a
4467-bp fragment whose nuclotide sequence was determined. E. coli does not naturally synthesize carotenoids but,
by using the carotenogenic genes recombinant strains accumulated ß-carotene where the four genes were expressed
as follows: farnesyl diphosphate (FPP) crtE geranylgeranyl diphosphate (GGPP) crtB phytoene crtI lycopene crtY
ß-carotene. E. coli BL21 acquired the yellow pigments due to the prescence of ß-carotene after transformed by
pGEX-5X-3-crtEBIY.
[Hany M. Yehia, Alaeldein M. Abudabos, Manal F. Elkhadragy, Ebtesam M. Al- Olayan, Abd-El-Rahman M.
Khalaf- Allah and Nagwa M. El-Shimi. Cloning, sequencing and expressing of the carotenoid biosynthesis genes
of ß-carotene from epiphytic bacteria Erwinia uredovora in non carotenogenic bacteria E. coli. Life Sci J 2013;
10(3): 1904-1917] (ISSN: 1097-8135). http://www.lifesciencesite.com. 283
Key Words: Erwinia uredovora, carotenoid genes, Eschershia coli BL21.

نوع عمل المنشور
published
مزيد من المنشورات
publications
بواسطة 3. -Krücken J, Mehnert LI, Dkhil MA, El-Khadragy M, Benten WP, Mossmann H, Wunderlich F (2005):
2005
publications
بواسطة 2. -Jürgen Krücken, Denis Delić, Heike Pauen, Anna Wojtalla, Manal El-Khadragy, Mohamed A Dkhil, Horst Mossmann and Frank Wunderlich
2009
publications
بواسطة 1Mohamed M.Fathi, Mohamed S.Gabry, Mona Abdel-Rahman and Manal F.El-khadragy (1999)
1999