Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers

مقال فى مجلة

2021

تم النشر فى: https://doi.org/10.1016/j.sjbs.2020.11.063

The existence of genetic diversity in Crocus sativus has globally remained a mystery till date. The study investigated PCR based DNA amplification profile of saffron using ISSR and RAPD based primers. A total of 38 amplicons were generated by ISSR primers in the range from 7 to 12 with an average of 9.50 bands per primer. 20 bands were found to be polymorphic and 18 were monomorphic with an average percentage of polymorphism as 52.48%. RAPD based amplification revealed a total 161 amplicons, 107 as polymorphic and 54 as monomorphic with an average percentage of polymorphism as 66.44%. Cumulative results of RAPD and ISSR demonstrated that Nei-Li's similarity index ranged between 0.70 and 0.97. The results of AMOVA has revealed 9% of variance among populations and 91% of variance within populations, Phi PT was found as 0.089, which indicates existence of genetic differences though limited. In conclusion, the results indicate that saffron accessions are minimally genetically differentiated, which could be capitalized in future breeding programmes to ameliorate this precious crop. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

• نوع عمل المنشور: Article
• رقم المجلد: 28
• رقم الانشاء: 2
• مجلة/صحيفة: SAUDI JOURNAL OF BIOLOGICAL SCIENCES