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د. محمد بن مفضي بن حمد العنزي Dr. Mohammed Mufadhe H. Alanazi

Assistant Professor

عضو هيئة تدريس

كلية الصيدلة
Building 23 (College of Dentistry), First floor, Office 1A 29
المنشورات
مقال فى مجلة
2018

Projection length stimulated by oxytocin is modulated by the inhibition of calcium signaling in U-87MG cells.

J., Zatkova M, Bacova Z, Puerta F, Lestanova Z, Alanazi M, Kiss A, Reichova A, Castejon AM, Ostatnikova D, Bakos . 2018

Neuropeptide oxytocin contributes to the regulation of glial cell morphology. The precise mechanisms, however, are not yet fully understood. In the present study, we have investigated whether an oxytocin-induced increase of intracellular calcium is required for cell extension in astrocyte-like U-87MG cells. Oxytocin (1 µM) significantly increased the length of the cell projections measured by the green-fluorescent protein labeled microtubule-associated protein after 48 h. The knockdown of oxytocin receptors (OXTR) in U-87MG cells prevented the elongation of the projections. Incubation of U-87MG cells in the presence of oxytocin, resulted in a significant increase of intracellular calcium, specifically blocked by the OXTR antagonist L-371,257. Both quercetin, which is a phosphoinositide 3-kinase inhibitor, and the phospholipase C inhibitor U-73122 reduced oxytocin-induced elevation of intracellular calcium concentration. Conversely, neither diltiazem, an L-type voltage-gated calcium channel blocker nor tetracaine, which is a blocker of the ryanodine receptors, showed an effect on intracellular calcium levels. Treatment of cells with quercetin, U-73122 and the voltage-gated calcium channel blockers cilnidipine, ω-agatoxin and mibefradil prevented the elongation of projections stimulated by oxytocin. Oxytocin treatment resulted in a significant increase in gene and protein expression of the scaffolding protein SHANK3. Our results clearly show that activation of OXTRs contributes to the elongation of cell projections in astrocyte-like U-87MG cells and that this effect is mediated by an extracellular calcium influx accompanied by an increase in scaffolding proteins expression.

رقم المجلد
125
رقم الانشاء
12
مجلة/صحيفة
Journal of Neural Transmission
الصفحات
1847-1856
مزيد من المنشورات
publications

Sepsis often leads to complications such as acute kidney injury (AKI) which is reported to range from 30 to 50% in critically ill patients. Dendritic (DCs) and neutrophils play a decisive role in…

بواسطة Al-Harbi NO, Nadeem A, Ahmad SF, Alanazi MM, Aldossari AA, Alasmari F1.
2019
publications

Neuropeptide oxytocin contributes to the regulation of glial cell morphology. The precise mechanisms, however, are not yet fully understood. In the present study, we have investigated whether an…

بواسطة Zatkova M, Bacova Z, Puerta F, Lestanova Z, Alanazi M, Kiss A, Reichova A, Castejon AM, Ostatnikova D, Bakos J.
2018
publications

Oxytocin is a neuropeptide widely expressed in the brain. Oxytocin plays a role in both proliferation and differentiation of various cells.

بواسطة Lestanova Z, Puerta F, Alanazi M, Bacova Z, Kiss A, Castejon AM, Bakos J
2017