DISPERSED GOLD NANOPARTICLES POTENTIALLY RUIN GOLD BARLEY YELLOW DWARF VIRUS AND ELIMINATE VIRUS INFECTIVITY HAZARDS
Alkubaisi, Noorah A. . 2016
Gold nanoparticles (AuNPs) application melted
barley yellow dwarf virus-PAV (BYDV-PAV) spherical
nanoparticle capsids. Synergistic therapeutic effects for
plant virus resistance were induced by interaction with
binding units of prepared AuNPs in a water solution which
was characterized and evaluated by zeta sizer, zeta potential
and transmission electron microscopy (TEM). The
yield of purified nanoparticles of BYDV-PAV was
obtained from Hordeum vulgare (Barley) cultivars, local
and Giza 121/Justo. It was 0.62 mg/ml from 27.30 g of
infected leaves at an A260/A280 ratio. Virus nanoparticle
has a spherical shape 30 nm in size by TEM. BYDV-PAV
combined with AuNPs to challenge virus function in vivo
and in vitro. Dual AuNPs existence in vivo and in vitro
affected compacted configuration of viral capsid protein in
the interior surface of capsomers, the outer surface, or
between the interface of coat protein subunits for 24 and
48 h incubation period in vitro at room temperature. The
sizes of AuNPs that had a potentially dramatic deteriorated
effect are 3.151 and 31.67 nm with a different intensity of
75.3% for the former and 24.7% for the latter, which
enhances optical sensing applications to eliminate virus
infectivity. Damages of capsid protein due to AuNPs on the
surface of virus subunits caused variable performance in
four different types of TEM named puffed, deteriorated and
decorated, ruined and vanished. Viral yield showed
remarkably high-intensity degree of particle symmetry and
uniformity in the local cultivar greater than in Giza
121/Justo cultivar. A high yield of ruined VLPs in the local
cultivar than Justo cultivar was noticed. AuNPs indicated
complete lysed VLPs and some deteriorated VLPs at 48 h.
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