تجاوز إلى المحتوى الرئيسي
User Image

Nabil MS Amor

Assistant Professor

Assistant Professor Bioinformatics

كلية العلوم
2B 158
المنشورات
مقال فى مجلة
2011

First report of Fasciola hepatica in Equus caballus host species from Tunisia based on the ribosomal internal transcribed spacer regions

Amor, Nabil . 2011

Fasciola hepatica Equus caballus Tunis PCR ITS genetic characterization

 Fasciolosis caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea) is considered the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems. Samples identifi ed morphologically as Fasciola sp. in Equus caballus host species from the city of Tunis (north of Tunisia) were genetically characterized, for the fi rst time, by sequences of the 1st (ITS-1), the 5.8S and 2nd (ITS-2) Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA). Comparison of the ITS of the Tunisian samples with sequences of Fasciola spp. from GenBank showed that the specimens examined had sequences identical to those of F. hepatica sequences selected as reference. Th e nucleotide sequencing of ITS rDNA showed no nucleotide variation in the ITS-1, 5.8S, or ITS-2 rDNA sequences among all F. hepatica samples parasitizing horses from Tunis, versus 2 ITS-2 haplotypes in standard F. hepatica, showing a substitution C/T in position 859, reported previously from Tunisia, Algeria, and Spain. Th e present study is the fi rst demonstration of the existence of the most frequent haplotype (FhITS-H1) of F. hepatica species in horses from Tunis (north of Tunisia) by the genetic approach using ITS rDNA as genetic marker, providing a foundation for further studies on Fasciola sp. in North African countries. 

رقم المجلد
5
رقم الانشاء
35
مجلة/صحيفة
Turk. J. Vet. Anim. Sci
الصفحات
319-324
مزيد من المنشورات
publications

A high percentage of camel handlers in Saudi Arabia are seropositive for Middle East respiratory syndrome corona- virus.

2019
publications

A high percentage of camel handlers in Saudi Arabia are seropositive for Middle East respiratory syndrome corona- virus.

2019
publications

Fasciolosis is a foodborne trematodosis characterised by a worldwide distribution. Various approaches have been developed for the study of the causative agents of this parasitic infection:…

2020