Effect of alpha lipoic acid on in vitro development of bovine secondary preantral follicles

Journal Article

• نوع عمل المنشور: paper
• رابط المنشور على الويب: theriogenology
• المجلة \ الصحيفة: theriogenology
• رقم العدد: in press
• رقم الإصدار السنوي: in press
• الصفحات: in press

مستخلص المنشور

The present study aimed to evaluate the in vitro effect of alpha lipoic acid (ALA) addition to the culture medium on the development of the bovine secondary preantral follicles. Bovine secondary preantral follicles were collected and divided into two groups depending on their size (80-100 μm and 100-110 μm). They were cultured an in vitro for 15 days (D) using different media including at three different doses of alpha lipoic acid (ALA). The genes expression levels of Follicle stimulating hormone beta-subunit (FSHR), insulin-like growth factor (IGF-1), Activin, Luteinizing hormone/choriogonadotropin receptor (LHCGR), Bone morphogenetic protein receptor type IA (BMPR1A), Transforming growth factor beta receptor 1 (TGFβR1), Growth differentiation factor 9 (GDF9), BCL2-associated X protein (BAX) and C-Myc were studied using real time polymerase chain reaction (RT-PCR). The protein expression levels of FSHR, IGF-1 and BAX were measured using western blot analysis. The results of the present work revealed that in vitro addition of ALA induced significant increase in the growth and development of secondary preantral follicles throughout the culture period as compared to control. The FSHR, IGF-1, LHCGR, BMPR1a, TGFβR1, GDF9 and Activin A genes were up regulated in ALA treated follicles as compared to the control. On contrary, pre-apoptotic genes BAX and C-Myc were down- regulated in treated follicles compared to control ones. The protein levels of FSHR and IGF-1 were highly expressed in treated follicles compared to control; however, BAX protein was down regulated in the treated follicles groups. The addition of ALA to the culture medium enhances secondary preantral follicles development and growth.