Improvement in natamycin production by Streptomyces natalensis with the addition of short-chain carboxylic acids

Journal Article

Enshasy, Elsayed EA, Mohamed Abdel Fattah Farid, Hesham Ali El . 2013

• المجلة \ الصحيفة: Process Biochemistry
• رقم العدد: 12
• رقم الإصدار السنوي: 48
• الصفحات: 1831-1838

مستخلص المنشور

Natamycin  is  an  important  tetraene  (polyene)  antibiotic  produced  in  submerged  culture  by  different strains  of  Streptomyces  sp.  In  the  present  work,  the  effects  of  the  addition  of  short-chain  carboxylic  acids (acetic,  propionic  and  butyric)  on  cell  growth  and  the  kinetics  of  natamycin  production  were  investigated during  submerged  cultivation  of  Streptomyces  natalensis.  The  addition  of  acetic  and  propionic  acids showed  stimulatory  effects  on  natamycin  production  when  added  to  the  fermentation  medium  at  concentrations  below  2  g  L−1 at  the  beginning  of  cultivation.  In  addition,  when  acetic  and  propionic  acids were  added  in  a  mixture  (7:1)  at  a  total  concentration  of  2  g  L−1 ,  antibiotic  production  increased  significantly,  reaching  3.0  g  L−1 (approximately  223%  and  250%  increases  in  volumetric  and  specific  antibiotic production,  respectively,  compared  with  the  control  culture).  Moreover,  the  addition  of  carboxylic  acids not  only  increased  the  antibiotic  yield  but  also  decreased  the  production  time  from  96  h  to  only  84  h in  shake-flask  cultures.  A  further  enhancement  in  natamycin  production  was  achieved  by  cultivation  in a  2-L  stirred-tank  bioreactor  under  controlled  pH  conditions.  The  maximum  volumetric  production  of 3.98  g  L−1 was  achieved  after  84  h  in  carboxylic  acid-supplemented  culture  (acetate  and  propionate  in  a ratio  of  7:1)