Effect of Inorganic Mercury on Drug Metabolizing Enzymes of Promethazine-Pretreated and Phenobarbitone–Pretreated Male Rabbit

Journal Article

Shakoori, Farah Anjum,1 Farah R. Shakoori,1 K.A. Al-Ghanim2 and Abdul Rauf . 2016

• نوع عمل المنشور: Inorganic mercury, drug metabolizing enzymes, promethazine, phenobarbitone, Cytochrome p450, Cytochrome b5, flavin monooxygenase system.
• رابط المنشور على الويب: Pakistan J. Zool
• الوسوم: Inorganic mercury, drug metabolizing enzymes, promethazine, phenobarbitone, Cytochrome p450, Cytochr
• المجلة \ الصحيفة: Pakistan J. Zool
• رقم العدد: 5
• رقم الإصدار السنوي: 48
• الصفحات: 1485-1494

مستخلص المنشور

Effects of inorganic mercury have been studied on the drug metabolizing enzymes (DME) of
phenobarbitone- (PB-) and promethazine- (PM-) pretreated male New Zealand white rabbit,
Oryctolagus cunciculus. After PB treatment, the activities of cytochrome p-450 (CP), aniline
hydroxylase (AH), acetanilide hydroxylase (AAH), benzphatamine demethylase (BD), aminopyrine
demethylase (APD) and N.N dimethyl aniline demethylase (DAD) increased 214, 266, 207, 108, 81
and 83%, respectively. Cytochrome-c-reductase (CCR) also increased 109%. However, the activities
of cytochrome b5 (CB) and N.N dimethyl aniline N-oxide (DAO) decreased 76% and 38%,
respectively. PM administration had no effect on any of the DMEs except for AH, AAH and BD,
which showed 60, 5 and 16% increase, respectively. HgCl2 administered at a dose of 1mg/kg body
wt per day for 5 days did not affect the CP, CB, APD and CCR. The activities of AH and AAH
increased 32 and 33%, respectively, whereas those of BD, DAD, and DAO decreased 35%, 30% and
32%, respectively. When administered to PB-pretreated animals CP, AH, AAH, BD, APD, DAD,
CCR, and DAO were inhibited 57, 58, 42, 31, 56, 55, 76 and 59%, respectively. Activity of CB
increased 351%. HgCl2 had no effect on CP and CCR when administered to PM-pretreated animals.
AH, AAH and CB increased 17, 135 and 30%, respectively. BD, APD and DAD were reduced 47,
10 and 19, respectively. Formation of DAO was also inhibited 52%. To conclude, mercury
drastically interferes with the DME. The metabolism of PB is inhibited, whereas that of PM is
variably affected – hydroxylases are up-regulated and demethylases are down-regulated. CB is
however, induced 351% after mercury treatment.