توزيع منج ٣٣٢ كلن مع المراجع

CLS 332:   Lectures Outline

 

Weeks                                               Subjects 

 

1.                                  Introduction

-      

Electromagnetic radiation

-      

Absorption spectroscopy

-      

Spectrophotometric techniques

-      

Application of Beer's law

-      

Visible absorption spectrophotometer,

-      

Components and applications

 

2.                                  Atomic absorption spectrophotometer

-      

Principle, basic components and their function

-      

Flamless atomic absorption spectrophotometer

-      

Advantages, interferences

 

3.                                  Emission spectroscopy

-      

Luminescence

-      

Fluorometers

-      

Fluorometers v/s conventional spectrophotometers

-      

Instrumentation

-      

Possible disadvantages, solutions

-      

Special application of fluorometers

 

4.                                  Light scattering phenomenon

-      

Turbidity and nephlometery

-      

Nephlomter v/s spectrophotometer

 

5.                                  Flame photometry

-      

Internal standard in flame photometry

-      

Advantages/disadvantages

 

6.                                 Osmometry

-      

Osmosis, colligative properties

-      

Osmolality/osmolarity

-      

Osmometers

-      

Freezing point osmometers

-      

Vapor presure osmometers

 

7.                                Ion selective electrodes

-      

Types of electrodes, principle and function

-      

Reference electrodes

-      

Glass pH electrodes

-      

Precipitate electrodes

-      

Solid state electrodes

-      

PCO2 and PO electrodes

 

8.                                Electrophoretic techniques

-      

Introduction

-      

Buffers, support materials

-      

Endosmosis

-      

Factors affecting the migration rate

-      

Clinical applications of electrophoresis

 

9.                                Chromatographic techniques

-      

General principles

-      

Physical basis of separation

-      

Adsorption (liquid- solid)

 

10.                              Partition (liquid- liquid)

-      

Gel filtration (steric exclusion)

-      

Ion exchange chromatography

 

11.                              Thin layer chromatography

-      

High performance liquid chromatography

-      

Gas chromatography

 

12.                              Automated procedures

-      

Basic concepts, operation, trouble shooting

-      

Automatic disponsors (dilutors)

-      

Types of automated equipment

-      

Continuos flow analyzers

-      

Discrete analysers

 

13.                              Single channel instruments

-      

Discontinious operation

-      

(Batch processing, single specimen)

-      

Multichannel instruments

-      

Small instruments….six channels

-      

Large instruments…non discretory, discretory

-      

Contrifugal analysers

 

References:

 

1. Daniel C. Harris, Quantitative Chemical Analysis, W.H. Freeman & Company; 5th edition (August 1998).

 

1. Burtis and Ashwood, Saunders, Teitz Fundamentals of Clinical Chemistry, 4^th edition, 2000