Series of Schiff bases of valproic acid hydrazide,

N

-valproylglycine hydrazide and

N

-valproyl-4-ami

-

nobenzoyl hydrazide derivatives were synthesized and characterized by IR, NMR (

1

H- and

13

C-NMR) and

elemental analysis. The prepared compounds were evaluated in transgenic zebrafish embryos (Tg:

flil-1

: en

-

hanced green fluorescent protein (EGFP)) for antiangiogenic activity and in HepG2 liver carcinoma cell line

for anti cancer activity. The Schiff bases of

N

Pulicaria genus belongs to the family Compositae. Some species of this genus have been used in traditional Arab medicine as antispasmodic agent. In this study the Pulicaria glutinosa, which is a wild growing shrub in Saudi Arabia have been explored for developmental toxicity using zebrafish embryos. The fractionated crude extract showed diverse biological activity as methanol fraction exhibited highest antioxidant activity (IC50 12.2?0.27%) followed by chloroform fraction (18.1?0.36%), while hexane fraction did not show any antioxidant activity.

Pulicaria is a genus of flowering plant in the Asteraceae family. It contains over 100 species. In this study the crude extract was prepared from the fresh leaves of P. glutinosa and further fractionated into three solvent to solvent fractions. The zebrafish (Danio rerio) embryos were treated with these extracts to explore the developmental toxicity. The crude extract did not show any significant toxicity; however, hexane fraction induced severe neurotoxicity in zebrafish embryos at 5 μg/mL. The brain formation was severely impeded and sever apoptosis was observed in treated embryos.

Background: Valproic acid (VPA) is a potent anticancer and antiangiogenic agent. However, design andsynthesis of chemical derivatives with improved antiangiogenic and anticancer activities are still necessary. Inthis study a library of novel derivatives of VPA was synthesized and tested.

Purpose:
To  investigate  the  antiproliferative  and  apoptotic  activity  of  crude  and  dichloromethane fraction of A. sieberi against seven cancer cell lines (Colo20, HCT116, DLD, MCF7, Jurkat, HepG2 andL929).
Methods: