The purpose of this study was to evaluate in vitro the antibacterial effect of calcium hydroxide points by using a broth dilution test. Extracts of calcium hydroxide points and a calcium hydroxide paste (Calasept) were tested by using 2 facultative anaerobic bacteria, Streptococcus mutans (ATCC 27352) and Enterococcus faecalis (ATCC 29212). Saline solution was used as a control. Each tested agent was kept in contact with the bacterial species used for the experiment for 5 minutes, 1 hour, 1 day, 2 days, and 5 days.

Purpose. The purpose of this investigation was to study in-vitro the attachment behavior of human periodontal ligament

(HPDL) fibroblasts to nondemineralized dentin surface using scanning electron microscope.

Study design. Thirty root slices of freshly extracted human teeth of 4 mm thickness as well as six 5 3 5 mm glass slides

used as a control were used in this study. The dentin surface of the root slices was not treated with any chemicals to

The purpose of this investigation was to study, in vitro, the antifungal effect of mineral trioxide aggregate (MTA) using a tube-dilution test. MTA was tested freshly mixed and after 24 h set on Candida albicans. The tested MTA was incubated with C. albicans for 1 h, 24 h, and 3 days.

A b s t r a c t
An immature maxillary first premolar in an 8-year-old female was treated using a regenerative approach. The root canal was
gently irrigated with 5.25% sodium hypochlorite without instrumentation under aseptic conditions and then medicated with
calcium hydroxide (Ca[OH]2) for 3 weeks. The Ca(OH)2 was removed, and bleeding was initiated mechanically using a hand
file to form an intracanal blood clot. Mineral trioxide aggregate was placed over the blood clot, and the access cavity was