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Dr. Quaiser Saquib

Professor

Professor

كلية العلوم
Building 5, 2B129 (Time: 9:00 AM- 4:00 PM)
المنشورات
مقال فى مجلة
2013

Phytotoxic hazards of NiO-nanoparticles in tomato: A study on mechanism of cell death

Musarrat, M. Faisal, Q. Saquib, A.A. Alatar, A.A. Al-Khedhairy, A.K. Hegazy, J. . 2013

Nickel oxide nanoparticles (NiO-NPs) in the concentration range of 0.025–2.0 mg/ml were examined for the induction of oxidative stress, mitochondrial dysfunction, apoptosis/necrosis in tomato seedling roots, as an in vivo model for nanotoxicity assessment in plants. Compared to the control, catalase (CAT), glutathione (GSH), superoxide dismutase (SOD) and lipid peroxidation (LPO) in 2.0 mg/ml NiO-NPs treatments exhibited 6.8, 3.7, 1.7 and 2.6-fold higher activities of antioxidative enzymes. At 2.0 mg/ml, 122% and 125.4% increase in intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) of seedling roots confirmed the oxidative stress and mitochondrial dysfunction. Comet assay exhibited a significant increase in the number of apoptotic (21.8%) and necrotic (24.0%) cells in 2.0 mg/ml treatment groups vis-á-vis in control 7% apoptotic and 9.6% of necrotic cells were observed. Flow cytometric analysis revealed 65.7% of apoptotic/necrotic cell populations and 2.14-fold higher caspase-3 like protease activity were recorded in 2.0 mg/ml treatment groups. Ultrastructure analysis revealed NiO-NPs translocation, nuclear condensation, abundance in peroxisomes and degenerated mitochondrial cristae. The dissolution of Ni ions from NiO-NPs signifies its potential to induce cell death presumably by Ni ions, triggering the mitochondrial dependent intrinsic apoptotic pathway.

نوع عمل المنشور
KSU Research Work
رقم المجلد
250–251
رقم الانشاء
15 April 2013
مجلة/صحيفة
Journal of Hazardous Materials
الصفحات
318–332
مزيد من المنشورات
publications

Tris(1,3-Dichloro-2-propyl)phosphate (TDCPP) is an organophosphorus flame retardant (OPFR) widely used in a variety of consumer products (plastics, furniture, paints, foams, and electronics).

بواسطة Quaiser Saquib, Abdullah M. Al-Salem, Maqsood A. Siddiqui, Sabiha M. Ansari, Xiaowei Zhang, Abdulaziz A. Al-Khedhairy
2022
publications

Tris (2-ethylhexyl) phosphate (TEHP) is an organophosphate flame retardant (OPFRs) which is extensively used as a plasticizer and has been detected in human body fluids.

بواسطة Quaiser Saquib, Abdullah M. Al-Salem, Maqsood A. Siddiqui, Sabiha M. Ansari, Xiaowei Zhang, Abdulaziz A. Al-Khedhairy
2022
publications

Recent reports have confirmed that tris(2-butoxyethyl) phosphate (TBEP), an organophosphorous flame retardants (OPFRs), profoundly detected in the dust from solid waste (SW), e-waste dumping sites…

بواسطة Quaiser Saquib, Abdullah M. Al-Salem, Maqsood A. Siddiqui, Sabiha M. Ansari, Xiaowei Zhang, Abdulaziz A. Al-Khedhairy
2022