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Reem A. Alajmi

Associate Professor

Vice dean of science collage

كلية العلوم
Bulding 5, 3rd floor, office # 5T005
المنشورات
مقال فى مجلة
2016

Molecular typing of phlebotomine sand flies in al-madinah and asir regions, Saudi Arabia using PCR–RFLP of 18S ribosomal RNA gene

Studies on the distribution of sand flies are important for the control of leishmaniasis in endemic and neighboring areas. In the present study polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) was used to identify the distribution of sand flies in Al-Madinah and Asir Regions of Saudi Arabia using PCR-RFLP of 18S ribosomal RNA gene. Based on the morphological characteristics, the sand flies were differentiated into seven species viz., Phlebotomus papatasi, Phlebotomus sergenti, Phlebotomus bergeroti, Sergentomyia clydei, Sergentomyia antennata, Sergentomyia fallax and Sergentomyia schwetzi. PCR-RFLP of 18S ribosomal RNA (rRNA) genes with eight different restriction enzymes resulted in species-specific agarose gel electrophoresis banding patterns. Of the eight restriction enzymes used, not a single restriction enzyme by itself could separate species belonging to the same genera (like P. papatasi and P. sergenti by AseI) as well as those belonging to different genera (like P. papatasi and S. clydei by AseI). We therefore conclude that the genetic diversity within sand fly species based on PCR-RFLP technique was nonspecific. Studies are in progress to study the viability of alternate techniques like low-stringency single specific primer polymerase chain reaction which can be used for molecular typing.

رقم المجلد
24
رقم الانشاء
7
مجلة/صحيفة
Saudi Journal of Biological Science
الصفحات
1697-1703
مزيد من المنشورات
publications
بواسطة Afnan S. Al-Qurashi, Ashraf M. Mashaly, Reem Alajmi, Mohamed S. Al-Khalifa, Lamjed Mansour, Suliman Y. Al-Omar, Mostafa R. Sharaf, Abdulrahman S. Aldawood, Hathal M. Al-Dhafer, Tony Hunter, Masha’el Y. Almutawa, Ashraf M. Ahmed
2023
تم النشر فى:
Journal of Medical Entomology
publications
بواسطة Hend M Alharbi, Yasser A Elnakady, Badr A Aldahmash, Reem Alajmi, Zeid A ALOthman, Ahmed-Yacine Badjah-Hadj-Ahmed, Ahmad Aqel, Ashraf M Ahmed
2023
تم النشر فى:
Journal of Medical Entomology
publications

Aedes aegypti (Culicidae), the mosquito that causes yellow fever, is regarded as a significant vector for
many disease agents. The current study sought to learn more about mosquito mid-gut…

بواسطة Sumaiah Al-Ghamdi, Mohamed A. Farrag, Rewaida Abdel-Gaber, Albandary Alrajeh, Saeed El-Ashram, Ommer Dafalla, Guillermo Tellez-Isaias, Mohammed Alkuriji , Reem Alajmi.
2023