Molecular characterization of camel, sheep and goat Echinococcus granulosus isolates in Riyadh, Saudi Arabia

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مستخلص المنشور: 

The  tapeworm, Echinococcus granulosus, causes a major public health as it larval stage causes the life threatening cyclozoonotic disease, in different parts of the world. E. granulosus were isolated from different livestock (camel, sheep and goat) of Riyadh in Saudi Arabia. The possibility of intra-genotype variation wasinvestigated using randomly amplified polymorphic DNA (RAPD) analysis by 20
random primers of 8-10 mers. After isolation of protoscolices from fertile cyst, DNA
was extracted and Internal Transcribed Spacer gene 1 (ITS1) was amplified using
specific primer and followed by Restriction Fragment Length Polymorphism (RFLP)
with the four restriction enzymes (HhaI, AluI, MspI & RsaI). Our study showed no
ITS1 variants could be detected and no genotypic variation among Saudi animal
isolates of E. granulosus on the basis of RAPD fingerprinting and RFLP-PCR.

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