Molecular detection and prevalence of Toxoplasma gondii in pregnant women in Sudan
Mohammed, Maha H. Elamin, Ebtesam M. Al-Olayan, Sawsan Ali Omer, Abdulaziz N. Alagaili, Osama B . 2012
The aim of the present study was to determine the prevalence of Toxoplasma gondii antibodies in
serum samples from two groups of women in Sudan and to use molecular techniques for detection of
the organism’s DNA using B1 gene. The first group (study group) composed of 94 individuals and they
aborted during the study, while the second group (control group) also composed of 94 individuals who
gave birth to healthy children without complications. Serological screening using enzyme linked
immunosorbent assay (ELISA) kits resulted in 39.4% and 35.1% of the women in the control and study
group showed positive IgG antibodies to T. gondii respectively. PCR amplification of B1 gene resulted
in detection of PCR product in 19.1% of the study group and 22.3% of the control group. There was no
association between positive toxoplasmosis antibodies and abortion, furthermore, the individuals with
positive PCR results in the control group did not abort. The usefulness of the B1 gene as a target for
toxoplasmosis diagnosis was documented and the possible source of infection in the control and the
study groups was discussed.
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