Resveratrol Reverses Thioacetamide-Induced Renal Assault with respect to Oxidative Stress, Renal Function, DNA Damage, and Cytokine Release in Wistar Rats
Background. Thioacetamide (TAA), a class 2B-type carcinogen, is a potent toxicant. Toxicities caused by this compound in various
tissues due to oxidative stress, increase of proinflammatory markers, and apoptosis have been reported; however, reports on kidney
toxicity are negligible. Resveratrol (RSV), on the other hand, has demonstrated antioxidant and anti-inflammatory effects in
different cases. Resveratrol’s protective effects against TAA kidney toxicity were investigated in four rat groups. Methodology.
Four groups of rats were studied as follows (n = 8): control group, where rats were fed normal diet and water; TAA group,
where rats received 0.3% TAA in water for two weeks; RSV group, where rats received 10 mg/kg body weight (bw) of RSV as
oral suspension for two weeks; and treated group, where rats orally received 10 mg/kg bw RSV and simultaneously received
0.3% TAA for two weeks. Kidney homogenates from all groups were analyzed for cytokine release (IL-4, TNF-α, and IFN-γ)
and oxidative stress (lipid peroxidation, catalase, and 8-OHdG). The serum of rats was analyzed for the quantification of renal
function markers (blood urea nitrogen (BUN), creatinine, and creatine kinase). Result. A significant increase in the renal
function markers (BUN, 240%; creatinine, 187%; and creatine kinase, 117%), oxidative stress parameters (lipid peroxidation,
192% increase; catalase, 30.5% decrease), cytokines (IL-4, 120%; TNF-α, 129%; and IFN-γ, 133%), and DNA damage was
observed in the TAA-treated group. All changes were significantly reversed in the group treated with RSV and TAA (P < 0 05)
in combination, with no significant difference compared to the control group. Conclusion. We conclude that resveratrol shows
protection against TAA toxicity in rat kidney with respect to DNA damage, oxidative stress, renal function and cytokine release.
