Betulinic Acid Potentiates Mast Cell Degranulation by Compromising Cell Membrane Integrity and Without Involving Fcεri Receptors

مقال فى مجلة

2024

تم النشر فى: https://pubmed.ncbi.nlm.nih.gov/38504489/

Mast cells play important role in acquired and natural immunity making
these favorable therapeutic targets in various inflammatory dis-
eases. Here we observed that, pentacyclic tri terpenoid betulinic acid 10
(BA) treatment resulted in a significantly high number (9%) of cells
positive for Hoechst and negative for annexin-V indicating that BA
could interfere with plasma membrane integrity. The degranulation of
both activated and non-activated mast cells was enhanced upon
treatment with BA. The pre-treatment of BA had remarkable effect 15
on calcium response in activated mast cells which showed increased
calcium influx relative compared to untreated cells. The results also
showed potentially less migration of BA treated mast cells signifying
the possible effect of BA on cell membrane. BA treatment resulted in
a significant increase in mRNA levels of IL-13 while as mRNA levels of 20
other target cytokines, IL-6 and TNF-α seem to be not affected.
Moreover, there was global Increase in phosphorylation of signaling
proteins and no significant change in phosphorylation of FcεRI receptors
indicating that the effect of BA was independent of signaling
cascade or FcεRI receptor mediated mast cell aggregation. Overall, 25
these results portray BA potentiates mast cell effector functions by
compromising the membrane integrity and independent of FcεRI
involvement.