Production of Anti-Camel IgY for diagnosis of infectious diseases affecting camels located in Kingdom of Saudi Arabia
Original article
Chicken can be used for the production of hyper-immune serum for many type of antigens specially, the
antigens of mammals and other species. Therefore, the current study aimed to use the chickens for the
productions of anti-camel IgY- antibodies using ammonium sulphate and ammonium sulphate - caprylic
acid methods for detection of any infectious disease affecting camels.
One ml of puried camel IgG of mixed with complete freund’s adjuvant was injected intramuscularly
and S/C to four groups of laying hens (Hisex strain, 140 days old). The rst booster dose was administrated
two week after rst immunization with one ml of puried camel IgG mixed with incomplete freund’s
adjuvant and the hens were repeatedly boostered (three times) at two weeks intervals. During the
immunization periods we collect the eggs every day and the anti-camel immunoglobulins Y was separated
by using two extraction methods; the ammonium sulphate precipitation method and the ammonium
sulphate caprylic acid method, the sensitivity and the specicity of the recovered antibody
against the camel IgG were evaluated using SDS-PAGE and ELISA.
Two weeks after the initial dose of immunizing the hens the anti-camels IgY-antibodies were detected
then the mean antibody titer increased signicantly (P < 0.05) till became the highest level at 10 weeks
post immunization. The antibody titer remain at high level without signicant increase (P > 0.05) up to
12 weeks after the rst dose of immunization then started to decrease.
Therefore, there are several benets of using immunized chicken to produce IgY antibodies rather than
using other species such as mammals in order to prepare anti-camels antibodies conjugates for diagnosis
of infectious disease affecting camels.
Chicken can be used for the production of hyper-immune serum for many type of antigens specially, the
antigens of mammals and other species. Therefore, the current study aimed to use the…