Skip to main content
User Image

Dr. rer. nat Elsayed Ahmed Elsayed

Associate Professor

Faculty Staff Member

كلية العلوم
Building 5, AB107
publication
Journal Article
2018

Effects of Different Aeration Rates and Feeding Strategies on Cell Growth and Invertase Production Kinetics by Saccharomyces boulardii

HA, Elsayed EA, El Enshasy . 2018

Invertase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) constitutes an improtant microbial enzyme with wide applications in differnt food and pharmaceutical sectors. The present work used the biotherapeutic yeast Saccharomyces boulardii to produce invertase under different aeration rates in stirred tank bioreatcr. Our results showed that an aeration rate of 1 v v-1 m-1 was the most suitable in terms of cell growth and invertase productivity. Highest enzyme production was recorded 14950 U L-1 after 50 h of cultivation. The production process was further optimized using different feeding strategties to overcome substrate limitation side effects encountered during batch cultivation. Sucrose feeding enhanced cell growth and enzyme productivity over batch cultivation by about 56.5% (from 7.35 to 11.5 g L-1) and 35.5% (from 14950 to 20250 U L-1), respectively. Furthermore, during feeding phase, invertase prodcution rate was improved by about 62.5% (from 299 to 486 U L-1 h-1), while growth rate remained constant. Additionally, the improved invertase production was mainly due to increased biomass and not cell productivity, since both batch and fed-batch cultivations have nearly similar specific growth values (2243.9 and 2194.1 U g-1, respectively). On the other hand, feeding complete medium greatly enhanced process parameters. Cell growth and invertase production increased from the batch cultivation by about 143.8 and 120.1% (17.92 g L-1 and 32900 U L-1, respectively), and from the sucrose-feeding cultivation by about 55.8 and 62.5%, respectively.

Volume Number
77
Issue Number
10
Magazine \ Newspaper
J. Sci. Ind. Res
Pages
575-582
more of publication
publications

Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4…

by El Enshasy, Elsayed EA, Suhaimi N, MalekRA, Esawy M
2018
publications

A series of azocompounds containing methyl salicylate 4a–k and 1-naphthyl moiety 6–8 was synthesized and tested as anticancer
agents. Nitrosation of methyl 5-amino-2-hydroxybenzoate or 1-…

by El-Naggar M, El-Shorbagi A-N, El-Naggar D, Amr AE, Al-Omar M, Elsayed EA
2018
publications

Invertase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) constitutes an improtant microbial enzyme with wide applications in differnt food and pharmaceutical sectors. The present work used…

by Elsayed EA, El Enshasy HA
2018