Course Description:
- Introduction to Histology, and its relation to other sciences. An idea to common terminology in histology
|
- Different methods in collecting tissue sample from an organism.
|
- The steps of routine histological preparations starting from fixation
|
- The objectives of fixation as the first step after obtaining tissue.
|
- Studying the different types of fixatives and give some examples for each type.
|
- Studying factors that affect fixation
|
- Studying different steps for preparation of histological slides (Tissue processor), including Dehydration ( The definition, the aim, and the chemical reagents which can be used)
|
- Studying Clearing and infiltration. ( The definition of each step, the aim of each step, and the chemical reagents which can be used in each step)
|
- To study the embedding step, its definition, the purpose of this step).
|
- Paraffin wax as a typical embedding material ( its chemical composition, its proprieties, methods to improve its properties).
|
- Comparing between the manual routine histological preparations and the automated histological preparation using tissue processor
machine. How to hold the tissue sections on glass slide.
|
- Staining the tissue. Different types of stains, and an example from
each type.Focusing on haematoxylin and eosin stain. Mounting
|
- Studying the structure of different microscopy ( light, phase- contrast, polarized and fluorescent), and compare between them.
|
- Studying the structure of electron microscope, giving an idea of how to prepare tissue to be visualized under electron microscope.
|
المرجع:
Basic Histology: Text & Atlas. (2005). Luiz Junqueira & Jose Carneiro
