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Dr. Taghreed Nasser Almanaa _ تغريد ناصر عبدالعزيز المانع

Assistant Professor

Faculty Member

كلية العلوم
Building 5, Floor 3, Office 202
publication
Journal Article
2021

Anti-biofilm effect of Nerium oleander essential oils against biofilm forming Pseudomonas aeruginosa on urinary tract infections

Taghreed, N. Almanaa, Naiyf S. Alharbi, Govindan Ramachandran, Chenthis Kanisha Chelliah, Govindan Rajivgandhi, Natesan Manoharan, Shine Kadaikunnan, Jamal M. Khaled, Khalid F. Alanzi . 2021

Based on the untreated biofilm formation in urinary tract infections (UTIs), current study was focused on the inhibition of Pseudomonas aeruginosa (P. aeruginosa) biofilm formation using Nerium oleander (N. ole- ander) essential oils. Initially, the LC-MS analysis of antibacterial potential of hydro distillated N. oleander essential oils was detected by LC-MS analysis. Initially, the antibacterial nature of essential oils was detected by agar well diffusion method against biofilm forming P. aeruginosa. Then, the possible anti- bacterial fractions of the essential oils were purified by preparative HPLC, and confirmed by analytical HPLC peaks. Possible anti-bacterial components of the fractions were merged together and confirmed by agar well diffusion method. Consecutively, the and minimum biofilm inhibition concentration exper- iment was proved that the concentration of 200 mg/mL of purified essential oils was very effective against P. aeruginosa biofilm formation. The decrease survival rate of the MTT assay result was also supported the minimum biofilm inhibition concentration result. Finally, all the results were more evident; the HPLC purified N. oleander essential oils as a promising anti-biofilm factor against P. aeruginosa biofilm formation. 

Volume Number
33
Magazine \ Newspaper
Journal of King Saud University - Science
Pages
101340
more of publication
publications

The antimicrobial agents of silver nanoparticles (AgNPs) have been applied a little while back in diverse therapeutic studies. In this analysis, AgNPs were biosynthesized using an ecologically…

by Rasha El-Mekkawy, Taghreed N. Almanaa, Marwa A. Yassin, Gamal Rabie, Noha Saleh
2021
publications

The current study investigates the capacity of a lipolytic Lactobacillus paracasei postbiotic as a possible regulator for lipid metabolism by targeting metabolic syndrome as a possibly…

by Ali Osman، Nashwa El-Gazzar،Taghreed N. Almanaa، Abdalla El‐Hadary and Mahmoud Sitohy
2021
publications

Based on the untreated biofilm formation in urinary tract infections (UTIs), current study was focused on the inhibition of Pseudomonas aeruginosa (P. aeruginosa) biofilm formation using Nerium…

by N. Almanaa, Naiyf S. Alharbi, Govindan Ramachandran, Chenthis Kanisha Chelliah, Govindan Rajivgandhi, Natesan Manoharan, Shine Kadaikunnan, Jamal M. Khaled, Khalid F. Alanzi Taghreed
2021