Intensification of resveratrol cytotoxicity, pro‑apoptosis, oxidant potentials in human colorectal carcinoma HCT‑116 cells using zein nanoparticles

مقال فى مجلة

2022

تم النشر فى: https://doi.org/10.1038/s41598-022-18557-2

Resveratrol (RSV), a non-flavonoid stilbene polyphenol, possesses anti-carcinogenic activities against
all the major stages of cancer. Zein nanoparticles (ZN NPs) have been utilized successfully in delivery
of variant therapeuticals by virtue of their histocompatible nature. The goal of this work was to
comparatively explore the antiproliferative, pro-apoptotic and oxidative stress potentials of RSV-ZN
NPs versus RSV against human colorectal carcinoma HCT-116 cells. ZN-RSV NPs were developed and
assayed for particle size analysis and RSV diffusion. The selected formula obtained 137.6 ± 8.3 nm
as mean particle size, 29.4 ± 1.8 mV zeta potential, 92.3 ± 3.6% encapsulation efficiency. IC50
of the
selected formula was significantly lower against HCT-116 cells versus Caco-2 cells. Also, significantly
enhanced cellular uptake was generated from RSV-ZN NPs versus free RSV. Enhanced apoptosis was
concluded due to increased percentage cells in G2-M and pre-G1 phases. The pro-apoptotic potential
was explained by caspase-3 and cleaved caspase-3 increased mRNA expression in addition to NF-κB
and miRNA125b decreased expression. Biochemically, ZN-RSV NPs induced oxidative stress as
demonstrated by enhanced reactive oxygen species (ROS) generation and endothelial nitric oxide
synthase (eNOS) isoenzyme increased levels. Conclusively, ZN-RSV NPs obtained cell cycle inhibition
supported with augmented cytotoxicity, uptake and oxidative stress markers levels in HCT-116 tumor
cells in comparison with free RSV. These results indicated intensified chemopreventive profile of RSV
due to effective delivery utilizing ZN nano-dispersion against colorectal carcinoma HCT-116 cells

• اسم الناشر: Scientific Reports