PHRM 213 Pharmaceutical analysis (1)
:Course Description
The course will include introduction to both volumetric and spectroscopic methods of analysis. The course will cover the basic principles of each method, mathematical treatment, laws, calculation, advantages, and limitations and finally, the applications of each method in the field of pharmaceutical analysis
No List of Topics Contact Hours
1 Introduction to Pharmaceutical Analysis, Validation of Analytical Methods 2
2 Acid-Base Titrations 6
- Theories of acids and bases
- pH of strong acids and bases, weak acids and bases and salts.
- Buffer solutions.
- Titration curves
- Acid-base indicators.
- Acid-base titrations in non-aqueous medium
3 Precipitation Titrations 2
- Principles of precipitimetric titrations
- Methods of precipitation titrations.
4 Complex-formation Titrations 2
- Principles of complexometric titrations
- Titration curves of complexometric titrations
5 Oxidation-Reduction Titrations 4
- Theories and definitions for of oxidation-reduction reactions
- Nernst equation and oxidation potential of redox system
- Redox titration curves.
- Detection of end point in redox titrations
6 Ultraviolet-visible spectrophotometry 4
- The electromagnetic spectrum
- Laws of light absorption
- Molecular absorption spectra
- Instrumentation of spectrophotometry
- Spectrophotometric method development and application in quantitative analysis
7 Fourier transforms infra-red spectroscopy 2
8 Molecular emission spectrophotometry 3
- Theory of light emission
- Fluorescence and phosphorescence and chemical structure
- Instrumentation of fluorometry
- Applications of fluorimetry in non-fluorescent compounds after their derivatization
9 Atomic absorption and emission spectrophotometry 3
- Principles of atomic absorption and emission spectrophotometry
- Instrumentation of atomic absorption and emission spectrophotometry
- Applications of atomic absorption and emission spectrophotometry
Total 28
Suggested Reading:
•Analytical Chemistry - Gary D. Christian, 7th edition.
•Quantitative Chemical Analysis – Daniel C. Harris 7th edition.
•Pharmaceutical Analysis – David G. Watson 4th edition.
Module Questions:
Exam 1
Short Answer
1-Analytical chemistry
The branch of chemistry that deal with the separation, identification and determination of components in a sample.
2-Difference between qualitative and quantitative analysis.
Qualitative / attempting to identify what materials are present in a sample.
Quantitative/ determining how much of a material is present in a sample.
Discuss and give example for using acid- base titration method for analysis
You should include in your answer
- How does the method work?
- Why does it use?
- Advantages and disadvantages?
- Endpoint and equivalence point?