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نواف بن مساعد العتيبي Nawaf Musaid Alotaibi

Demonstrator

عضو هيئة تدريس Demonstrator, College of Medicine, Faculty of Internal Medicine, Pulmonology Unit

كلية الطب
مبنى كلية الطب، الدور الثاني، قسم الباطنة

Phenotyping and outcomes of hospitalized COPD patients using rapid molecular diagnostics on sputum samples

Alotaibi, Nawaf M . 2019

COPD Molecular pathogen detection

Background: Etiologies of acute exacerbations of chronic obstructive pulmonary disease (AECOPD) are heterogeneous. We phenotyped severe AECOPD based on molecular pathogen detection of sputum samples collected at hospitalization of COPD patients and determined their outcomes.
Methods: We phenotyped 72 sputum samples of COPD patients who were hospitalized with a primary diagnosis of AECOPD using a molecular array that detected common bacterial and viral respiratory pathogens. Based on these results, the patients were classified into positive or negative pathogen groups. The pathogen-positive group was further divided into virus or bacteria subgroups. Admission day 1 blood samples were assayed for N-terminal prohormone brain natriuretic peptide, CRP, and complete blood counts.
Results: A total of 52 patients had a positive result on the array, while 20 patients had no pathogens detected. The most common bacterial pathogen detected was Haemophilus influenzae and the most common virus was rhinovirus. The pathogen-negative group had the worse outcomes with longer hospital stays (median 6.5 vs 5 days for bacteria-positive group, P=0.02) and a trend toward increased 1-year mortality (P=0.052). The bacteria-positive group had the best prognosis, whereas the virus-positive group had outcomes somewhere in between the bacteria-positive and pathogen-negative groups.
Conclusion: Molecular diagnostics on sputum can rapidly phenotype serious AECOPD into bacteria-, virus-, or pathogen-negative groups. The bacteria-positive group appears to have the best prognosis, while pathogen-negative group has the worst. These data suggest that AECOPD is a heterogeneous event and that accurate phenotyping of AECOPD may lead to novel management strategies that are personalized and more precise.

Magazine \ Newspaper
International Journal of Chronic Obstructive Pulmonary Disease
more of publication
publications

Background: Etiologies of acute exacerbations of chronic obstructive pulmonary disease (AECOPD) are heterogeneous. We phenotyped severe AECOPD based on molecular pathogen…

by Nawaf M Alotaibi, Virginia Chen, Zsuzsanna Hollander, Jonathon A Leipsic , Cameron J Hague, Darra T Murphy, Mari L DeMarco, J. Mark FitzGerald, Bruce M McManus, Raymond T Ng, Don D Sin
2019
publications

Rationale: Lung dysbiosis promotes airway inflammation and decreased local immunity, potentially playing a role in the pathogenesis of acute exacerbations of COPD (AECOPD). We determined the…

by Fernando Sergio Leitao Filho, Nawaf M Alotaibi, David Ngan, Sheena Tam , Julia Yang, Zsuzsanna Hollander, Virginia Chen, J. Mark FitzGerald, Corey Nislow, Janice M leung, SF Paul Man, Don D Sin
2018
publications

Introduction: The use of beta-blockers in chronic obstructive pulmonary disease (COPD) is controversial, primarily due to concerns that they may worsen lung function and attenuate bronchodilator…

by Nawaf M Alotaibi, Fernando Sergio Leitao Filho, Kei Yamasaki, David A Ngan, Don D Sin
2018